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1CarMeN Laboratory, INSERM U1060, INRA 1397,University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon,Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine,University of Geneva
Chapters
- 00:05Title
- 00:34Cell Culture Media and Solution Preparation
- 02:45Culture of Human Primary Muscle Cells
- 04:19Insulin Stimulation and Glucose Uptake
- 05:30Cell Lysis
- 06:06Determination of Radiolabeled Glucose
- 06:52Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
- 07:37Conclusion
En este método, las células musculares primarias humanas se cultivan in vitro para obtener miotubos diferenciados y se miden las tasas de captación de glucosa. Proporcionamos un protocolo detallado para cuantificar las tasas en los estados basales y estimulados con insulina usando [3H] 2-desoxi-D-Glucosa radiomarcada.
