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Real-time iontophorese med tetramethylammonium til kvantificering af volumen fraktion og tortuosity af hjernens ekstracellulære rum
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1Department of Medicine,University of Virginia, 2Department of Cell Biology,SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program,SUNY Downstate Medical Center, 4Division of Neonatology,University of Virginia, 5Department of Neuroscience and Physiology,New York University School of Medicine
Chapters
- 00:05Title
- 01:01Real-time Iontophoresis in Agarose
- 03:59Agarose Data Analysis
- 05:30Real-time Iontophoresis in Brain Slices
- 07:02Brain Data Analysis, Checking Transport Number and ISM Calibration
- 08:19Results: Curve Fitting Data in Agar and in Brain
- 09:21Conclusion
Denne protokol beskriver realtids-iontoforese, en metode, som måler fysiske parametre for hjernens ekstracellulære rum (ECS). Diffusionen af et inert molekyle, der frigives i ECS, anvendes til at beregne ECS-volumenfraktionen og tortuositeten. Det er ideelt til at studere akut reversible ændringer i hjernens ECS.
