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用于维持和区分生物型的实验室技术<em>霍乱弧菌</em>临床和环境隔离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
DOI:

07:58 min

May 30, 2017

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Chapters

  • 00:05Title
  • 01:28Preparation and Use of Frozen Stock Cultures
  • 02:46Growth of Overnight Cultures in Liquid Medium
  • 03:23Phenotypic Assays for Biotype Classification via Spotting
  • 04:04Motility Assay
  • 04:48Voges-Proskauer (VP) Assay
  • 05:47Results: Reliable Distinction Between Biotypes
  • 07:08Conclusion

Summary

Automatic Translation

该手稿除了进行一系列生物化学测定之外,还描述了适用于霍乱弧菌的维护技术,它们共同用于临床和环境V之间的快速可靠的分化。 霍乱弧菌生物型实验室设置。

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