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Laboratorieteknikker anvendt til at opretholde og differentiere biotyper af<em> Vibrio cholerae</em> Kliniske og miljømæssige isolater
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
DOI:

07:58 min

May 30, 2017

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Chapters

  • 00:05Title
  • 01:28Preparation and Use of Frozen Stock Cultures
  • 02:46Growth of Overnight Cultures in Liquid Medium
  • 03:23Phenotypic Assays for Biotype Classification via Spotting
  • 04:04Motility Assay
  • 04:48Voges-Proskauer (VP) Assay
  • 05:47Results: Reliable Distinction Between Biotypes
  • 07:08Conclusion

Summary

Automatic Translation

Dette manuskript beskriver egnede Vibrio cholerae vedligeholdelsesteknikker ud over en række biokemiske analyser, der kollektivt anvendes til hurtig og pålidelig differentiering mellem klinisk og miljømæssig V. Kolerae-biotyper i en laboratorieindstilling.

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