Journal
/
Bioengineering
/
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

DOI:
10.3791/55769-v

•

11:20 min

•

August 30, 2017

•

, , , , ,
1Institute of Chemistry,Academia Sinica, 2Department of Chemistry,National Taiwan University, 3Department of Materials and Mineral Resources Engineering,National Taipei University of Technology

Chapters

  • 00:05Title
  • 01:33Fluorimeter Setup for Upconversion Spectrum Measurement and Microscope Setup
  • 03:29Characterization of Caged-PKA and Caged PKA/UCNP Constructs and Photolysis Setup
  • 04:55Cell Sample Preparation and Microinjection of Caged-PKA/UCNP Complexes
  • 07:35Photoactivation of Caged-PKA/UCNP Using NIR Light in Living Cells Experiment
  • 08:33Results: Visualize PKA Signal Transduction Activation via NIR Photoactivation
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.

Tags

Keywords: Upconversion NanoparticlesPhotoactivationKinaseNear-infraredSignal TransductionFluorescence SpectrometerOptical Setup

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55769?article_type=v

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
check_url/55769?article_type=v

An Experimental System to Study Mechanotransduction in Fetal Lung Cells
check_url/55769?article_type=v

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
check_url/55769?article_type=v

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
check_url/55769?article_type=v

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
check_url/55769?article_type=v

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
check_url/55769?article_type=v

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
check_url/55769?article_type=v

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
check_url/55769?article_type=v

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
check_url/55769?article_type=v

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Read Article