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Journal
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Neuroscience
/
在成年小鼠中的亚型腺相关病毒9(AAV9)载体递送
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
Please note that all translations are automatically generated.
Click here for the English version.
在成年小鼠中的亚型腺相关病毒9(AAV9)载体递送
DOI:
10.3791/55770-v
•
08:32 min
•
July 13, 2017
•
Takahiro Tadokoro
,
Atsushi Miyanohara
,
Michael Navarro
,
Kota Kamizato
,
Stefan Juhas
,
Jana Juhasova
,
Silvia Marsala
,
Oleksandr Platoshyn
,
Erik Curtis
,
Brandon Gabel
,
Joseph Ciacci
,
Nada Lukacova
,
Katarina Bimbova
,
Martin Marsala
4
1
Neuroregeneration Laboratory, Department of Anesthesiology
,
University of California, San Diego
,
2
Institute of Animal Physiology and Genetics
,
Czech Academy of Sciences
,
3
Department of Neurosurgery
,
University of California, San Diego
,
4
Institute of Neurobiology
,
Slovak Academy of Sciences
Chapters
00:05
Title
01:19
Exposing the Pial Membrane
03:58
Opening the Pial Membrane and Insering the Subpial Needle for AAV9 Delivery
06:11
Results: GFP-expression in Spine Parenchyma and Brain Following Subpial AAV9 Delivery
07:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究的目的是通过在成年小鼠中使用新的唾液基因传递技术来开发和验证脊髓腺相关病毒9(AAV9)介导的基因递送的效力和安全性。
Tags
Subpial
AAV9
Vector Delivery
Spinal Cord
Spinal Gene Expression
Spinal Neurodegenerative Disorders
Amyotrophic Lateral Sclerosis
Spinal Traumatic Injury
Chronic Pain
Transgene Expression
Spinal Gray Matter
Spinal White Matter
Spinal Segments
Dura-opening
Subpial Needle
Anesthesia
Isoflurane
Paravertebral Muscle
Spinal Laminectomy
Dura Incision
Cervical
Occipital Bone
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