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Protocols for C-Brick DNA Standard Assembly Using Cpf1
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JoVE Journal Genetics
Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

DOI:
10.3791/55775-v

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12:03 min

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June 15, 2017

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, ,
1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences,Chinese Academy of Sciences

Chapters

  • 00:05Title
  • 01:01Preparation of crRNA
  • 03:00Construction of C-Brick Parts
  • 06:11C-Brick Assembly
  • 10:07Assembly of 3 Chromoprotein Cassetts Using a C-Brick Standard
  • 11:15Conclusion

Summary

Automatic Translation

Automatic Translation

CRISPR-associated protein Cpf1 can be guided by a specially designed CRISPR RNA (crRNA) to cleave double-stranded DNA at desired sites, generating sticky ends. Based on this characteristic, a DNA assembly standard (C-Brick) was established, and a protocol detailing its use is described here.

Tags

C-BrickDNA AssemblyCpf1CRISPRSynthetic BiologyDNA ConstructionChromoproteinPCRRNA Transcription

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