Journal
/
Developmental Biology
/
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

DOI:
10.3791/55792-v

•

12:38 min

•

September 20, 2017

•

, , , ,
1Department of Biological Sciences,University of Maryland, Baltimore County

Chapters

  • 00:05Title
  • 00:45Fixation of Staged Embryos
  • 01:52Sectioning for Immunolabeling
  • 04:04Confocal Imaging
  • 05:17Quantifying Microtubules
  • 07:10De Novo MT Assembly Assay
  • 09:05Depolymerize Existing MTs
  • 10:26Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
  • 12:00Conclusion

Summary

Automatic Translation

Automatic Translation

Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

Tags

Keywords: ImmunolabelingMicrotubulesZebrafish EmbryoDevelopmental NeurobiologyCell PolarityStable MicrotubulesDynamic MicrotubulesNascent MicrotubulesFixationSectioningImmunostainingPrimary AntibodiesSecondary AntibodiesDAPI

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55792?article_type=v

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
check_url/55792?article_type=v

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
check_url/55792?article_type=v

Microbead Implantation in the Zebrafish Embryo
check_url/55792?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/55792?article_type=v

Imaging Subcellular Structures in the Living Zebrafish Embryo
check_url/55792?article_type=v

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
check_url/55792?article_type=v

AFM and Microrheology in the Zebrafish Embryo Yolk Cell
check_url/55792?article_type=v

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons
check_url/55792?article_type=v

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)
check_url/55792?article_type=v

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Read Article