Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Use of Immunolabeling to Analyze Stable, Dynamic, …
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
 

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Article DOI: 10.3791/55792-v • 12:38 min • September 20th, 2017
September 20th, 2017 •
*1, *1, 1, 1, 1
1Department of Biological Sciences, University of Maryland, Baltimore County
* These authors contributed equally

Chapters

Summary
Automatic Translation

Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

Tags

Keywords: Immunolabeling Microtubules Zebrafish Embryo Developmental Neurobiology Cell Polarity Stable Microtubules Dynamic Microtubules Nascent Microtubules Fixation Sectioning Immunostaining Primary Antibodies Secondary Antibodies DAPI
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter