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在细胞分化过程中光介导的可逆调节丝裂原活化蛋白激酶途径<em>爪蟾</em>胚胎发育
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
DOI:

09:32 min

June 15, 2017

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Chapters

  • 00:05Title
  • 01:22Optogenetic Induction of Protein Localization in BHK21 Mammalian Cell Culture
  • 04:10Construction of an LED Array for Long-term Light Stimulation in a CO2 Incubator
  • 05:05Optogenetic Induction of PC12 Cell Differentiation
  • 06:04Optogenetic Control of Kinase Activity in Xenopus Embryos
  • 07:22Results: Optogenetic Induction of PC12 Cell Differntiation and Kinase Activity in Live Xenopus Embryos
  • 09:00Conclusion

Summary

Automatic Translation

该协议描述了在细胞分化和非洲爪蟾胚胎发育过程中调节丝裂原活化蛋白激酶(MAPK)活性的光遗传策略。这种方法允许哺乳动物细胞培养物和多细胞活生物体如非洲爪蟾胚胎中具有高空间和时间分辨率的可逆激活MAPK信号通路。

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