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Lysmedieret reversibel modulering af den mitogenaktiverede proteinkinasevej under celledifferentiering og<em> Xenopus</em> Embryonudvikling
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Developmental Biology
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JoVE Journal Developmental Biology
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Lysmedieret reversibel modulering af den mitogenaktiverede proteinkinasevej under celledifferentiering og<em> Xenopus</em> Embryonudvikling

DOI:
10.3791/55823-v

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09:32 min

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June 15, 2017

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, , , , , , ,
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences,University of Illinois at Urbana-Champaign, 3Neuroscience Program,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Chapters

  • 00:05Title
  • 01:22Optogenetic Induction of Protein Localization in BHK21 Mammalian Cell Culture
  • 04:10Construction of an LED Array for Long-term Light Stimulation in a CO2 Incubator
  • 05:05Optogenetic Induction of PC12 Cell Differentiation
  • 06:04Optogenetic Control of Kinase Activity in Xenopus Embryos
  • 07:22Results: Optogenetic Induction of PC12 Cell Differntiation and Kinase Activity in Live Xenopus Embryos
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en optogenetisk strategi til modulering af mitogenaktiveret proteinkinase (MAPK) aktivitet under celledifferentiering og Xenopus embryonal udvikling. Denne metode muliggør reversibel aktivering af MAPK-signalvejen i pattedyrcellekultur og i multicellulære levende organismer, som Xenopus- embryoner, med høj rumlig og tidsmæssig opløsning.

Tags

Keywords: Light-mediatedReversible ModulationMitogen-activated Protein Kinase PathwayCell DifferentiationXenopus Embryonic DevelopmentOptogenetic InductionCIBN-CRY2 InteractionFluorescence ImagingPHK21 CellsCRY2-mCherry-Raf1 Plasmid

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