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Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
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JoVE Journal Neuroscience
Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

DOI:
10.3791/55830-v

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11:56 min

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June 16, 2017

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1Department of Molecular Biology,Chonbuk National University

Chapters

  • 00:05Title
  • 00:48Preparation of Late-stage Embryos for Immunostaining
  • 04:19LacZ Staining and Immunostaining
  • 07:20Preparing Embryos to Image the ISN Nerves in Abdominal Segments 7 to A9
  • 10:03Results: Intersegmental Nerve Bundle (ISNb) Innervation at A3 and A4
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.

Tags

Keywords: Axonal Projection PatternMotor NeuronsDrosophilaNeural DevelopmentMotor Axon GuidanceNeural Subcircuit FormationVisualizationEmbryosPBT SolutionDechorionationDevitellinizationLacZ StainingX-gal Staining Solution

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