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体外生物荧光法对乳腺上皮细胞昼夜节律的表征
JoVE Journal
Genetics
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JoVE Journal Genetics
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
DOI:

11:56 min

September 28, 2017

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Chapters

  • 00:05Title
  • 00:53Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
  • 03:06Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
  • 05:28Transient Transfection of Cells with the Circadian Vector
  • 07:46Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
  • 08:52Results: Effects of Chemicls on the Cellular Biological Clock
  • 10:16Conclusion

Summary

Automatic Translation

一个体外生物荧光法测定乳腺上皮细胞的细胞昼夜节律。该方法利用哺乳动物细胞报告质粒表达不稳定的荧光在周期 2基因启动子的控制下。它可以适应其他细胞类型, 以评估器官特定的影响昼夜节律。

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