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Chapters
- 00:05Title
- 00:53Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
- 03:06Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
- 05:28Transient Transfection of Cells with the Circadian Vector
- 07:46Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
- 08:52Results: Effects of Chemicls on the Cellular Biological Clock
- 10:16Conclusion
En in vitro- bioluminescens analyse til at bestemme cellulære døgnrytme i Brystkirtlerne epitelceller er præsenteret. Denne metode udnytter pattedyr celle reporter plasmider udtrykker destabiliseret luciferase under kontrol af periode 2 gen promotor. Det kan tilpasses til andre celletyper til at evaluere orgel-specifikke virkninger på døgnrytme.
