A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:53Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
- 03:06Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
- 05:28Transient Transfection of Cells with the Circadian Vector
- 07:46Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
- 08:52Results: Effects of Chemicls on the Cellular Biological Clock
- 10:16Conclusion
En in vitro- Mareld analys att bestämma cellulära dygnsrytmen i mjölkkörtlar epitelceller presenteras. Denna metod använder tredjeparts däggdjursceller reporter plasmider uttrycker destabiliserat luciferas under kontroll av PERIOD 2 gen arrangören. Det kan anpassas till andra celltyper att utvärdera organ-specifika effekter på dygnsrytmen.
