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微流控成像流式细胞仪通过不对称检测时间延伸光学显微镜（ATOM）
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JoVE Journal Engineering
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
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微流控成像流式细胞仪通过不对称检测时间延伸光学显微镜（ATOM）

DOI:
10.3791/55840-v

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07:19 min

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June 28, 2017

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, , , , , , , ,
1Department of Electrical and Electronic Engineering,The University of Hong Kong, 2Department of Mechanical Engineering,The University of Hong Kong

Chapters

  • 00:05Title
  • 00:51ATOM System Setup
  • 03:20Data Acquisition and Analysis
  • 04:56Results: Single-cell Imaging of Mammalian Cells and Phytoplanktons with ATOM
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了用于超快速微流体流中单细胞成像的不对称检测时间拉伸光学显微镜系统的实现及其在成像流式细胞术中的应用。

Tags

MicrofluidicImaging Flow CytometryAsymmetric-detectionTime-stretch Optical MicroscopyATOMLabel-freeHigh ContrastSingle-cell ImagingUltra-fast Microfluidic FlowFiber CollimatorBroadband Femtosecond/picosecond Near-IR Pulsed LaserSingle Mode Dispersive Optical FiberOptical AmplifierTransmission Diffraction GratingTelescopic Relay Lens4f Imaging SystemObjective LensMicrofluidic ChipBeam SplitterFiber CouplerPhotodetectorOscilloscope

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