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Immunology and Infection
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Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

DOI:
10.3791/55842-v

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08:38 min

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May 15, 2017

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, ,
1Department of Ecophysiology,Max Planck Institute for Terrestrial Microbiology

Chapters

  • 00:05Title
  • 01:14Induction of Swarmer Cell Differentiation
  • 03:39Preparation of V. parahaemolyticus Swarmer Cells for Fluorescence Microscopy
  • 06:11Results: Single Cell Analysis Reveals Changes in Intracellular Localization of YFP-CheW During V. parahaemolyticus Differentiation
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol enables single cell microscopy of the differentially distinct Vibrio parahaemolyticus swimmer and swarmer cells. The method produces a population of swarmer cells easily available for single cell analysis and covers preparation of cell cultures, induction of swarmer differentiation, sample preparation, and image analysis.

Tags

Keywords: Vibrio ParahaemolyticusCellular DifferentiationSwarmer CellsSingle Cell ImagingFluorescence MicroscopyCell CycleCell MorphologyIntracellular Organization22′-BipyridylCalcium ChlorideHeart Infusion Agar

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