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诱导细胞分化和单细胞成像<em>副溶血性弧菌</em游泳者和Swarmer细胞
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
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诱导细胞分化和单细胞成像<em>副溶血性弧菌</em游泳者和Swarmer细胞

DOI:
10.3791/55842-v

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08:38 min

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May 15, 2017

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, ,
1Department of Ecophysiology,Max Planck Institute for Terrestrial Microbiology

Chapters

  • 00:05Title
  • 01:14Induction of Swarmer Cell Differentiation
  • 03:39Preparation of V. parahaemolyticus Swarmer Cells for Fluorescence Microscopy
  • 06:11Results: Single Cell Analysis Reveals Changes in Intracellular Localization of YFP-CheW During V. parahaemolyticus Differentiation
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

该协议允许单细胞显微镜检查差异不同的副溶血弧菌游泳运动员和swarmer细胞。该方法产生容易获得单细胞分析的swarmer细胞群，并涵盖细胞培养物的制备，诱导分子分化，样品制备和图像分析。

Tags

Vibrio ParahaemolyticusCellular DifferentiationSwarmer CellsSingle Cell ImagingFluorescence MicroscopyCell CycleCell MorphologyIntracellular Organization22′-BipyridylCalcium ChlorideHeart Infusion Agar

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