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从孤立的大鼠心脏的间质渗出物收集的倒置心脏模型

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JoVE Journal Medicine

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

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从孤立的大鼠心脏的间质渗出物收集的倒置心脏模型

DOI:

10.3791/55849-v

•

07:59 min

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June 20, 2017

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Kezhe Tan*^1,2, Zhaoping Ding*^1,2, Bodo Steckel, Sonja Hartwig, Stefan Lehr, Xiaoming Deng, Jürgen Schrader

¹Department of Anesthesiology, Changhai Hospital,Second Military Medical University, ²Department of Molecular Cardiology,Heinrich Heine University, ³Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center,Heinrich Heine University

Chapters

00:05Title
01:08Preparation of the Latex Cap and Intraventricular Balloon
03:06Isolation and Cannulation of the Heart
04:34Reversed-heart Model
06:24Results: Protein and Purine Analysis of Cardiac Transudate
07:33Conclusion

Summary

Automatic Translation

该方案描述了从分离的灌注大鼠心脏中收集心脏间质液的方法。为了将间质渗出物与冠状静脉流出物灌注液物理分离，Langendorff灌注的心脏被倒置，并且使用软胶乳帽收集在心脏表面上形成的渗出物（间质液体）。

Tags

Inverted Heart Model Interstitial Transudate Collection Isolated Rat Heart Paracrine Factors Interstitial Fluid Space Cardiac Cells Inflammation Regeneration Infarcted Myocardium Latex Heart Cover Mold Liquid Latex Silicon Tubing Cardiac Transudate Ventricular Balloon Pressure Transducer Langendorff Perfusion System

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