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Tuning i Hippocampal Theta Band<em> In Vitro</em>: Metoder til optagelse fra den isolerede gnagere Septohippocampal Circuit
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Chapters
- 00:05Title
- 00:54Isolating the Hippocampus
- 03:47Set Up Fast Perfusion for Recording the Isolated Hippocampus
- 04:34Extracellular Recording on In Vitro Hippocampal Theta Oscillations
- 07:57Optogenetic Control in the Isolated Hippocampus
- 09:11Results: Cell-type Specific Activity of Pyramidal Cell and PV Interneuron During Spontaneous Theta Oscillations in the Intact Hippocampal Preparation from a PV-TOM Mouse
- 10:19Conclusion
Her præsenterer vi en protokol til optagelse af rytmisk neuronalt netværk theta og gamma oscillationer fra et isoleret hele hippocampal præparat. Vi beskriver de eksperimentelle trin fra udvindingen af hippocampus til detaljer af felt-, enheds- og helcelle-patch-klemmeoptagelser samt optogenetisk pacing af theta-rytmen.
