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Kemo-enzymatisk syntese af N- glycans for arrayet udvikling og HIV antistof profilering
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Chemistry
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JoVE Journal Chemistry
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Kemo-enzymatisk syntese af N- glycans for arrayet udvikling og HIV antistof profilering

DOI:
10.3791/55855-v

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11:08 min

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February 05, 2018

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, , ,
1Genomics Research Center,Academia Sinica

Chapters

  • 00:04Title
  • 01:23Preparation of Glycans with Phosphonic Acid Tail
  • 04:12Glycan Array
  • 08:18Results: Synthesis of N-glycans and Development of Arrays for HIV Antibody Profiling
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

En modulopbygning til syntese af N- glycans for vedhæftet fil i en aluminium oxid-belagt glas glide (ACG dias) som en glycan microarray er blevet udviklet og dets anvendelse til profilering af en HIV bredt neutraliserende antistoffer er blevet påvist.

Tags

Chemo-enzymatic SynthesisN-glycansGlycan MicroarrayHIV Antibody ProfilingGlycobiologyCell Virus Specific GlycansGlycoarrayInfluenza DiagnosisCancer DiagnosisSerum AntibodiesGlycan SpecificitiesPhosphonic Acid LinkerPolyacrylamide GelTLCCerium Ammonium MolybdatePalladium HydroxideHydrogen Atmosphere

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