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Spinal Cord Neurons Isolation and Culture from Neonatal Mice
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JoVE Journal Neuroscience
Spinal Cord Neurons Isolation and Culture from Neonatal Mice

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

DOI:
10.3791/55856-v

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07:49 min

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July 11, 2017

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, , ,
1Department of Surgery, Division of Cardiothoracic Surgery,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Title
  • 00:58Harvesting Spinal Cords
  • 02:27Isolating Neurons
  • 05:53Results: Immunofluorescent Staining of Neurons Using Neuronal Cytoskeletal Marker – MAP2
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.

Tags

Spinal Cord NeuronsNeuron IsolationNeuron CultureNeonatal MiceDensity GradientTriturationNeuron ExtractionNeurobiology Experiments

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