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Meten van Synaptic vesikel endocytose in gekweekte Hippocampal neuronen
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
DOI:

07:30 min

September 04, 2017

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Chapters

  • 00:05Title
  • 00:40Culture Stimulation
  • 01:35Fixation and Staining
  • 03:42Epoxy Embedding
  • 05:43Results: Images of Presynaptic Terminal in Stimulated Neurons with High Potassium
  • 06:49Conclusion

Summary

Automatic Translation

Synaptic vesikel endocytose is gedetecteerd door de lichte microscopie van pHluorin gefuseerd met synaptic vesikel eiwit en elektronenmicroscopie van vesikel opname.

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