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Neuroscience

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JoVE Journal Neuroscience
High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
 

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay

Article DOI: 10.3791/55892-v • 10:30 min • November 5th, 2017
November 5th, 2017 •
*1, *1, 2, 3, *1, *1
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 3Department of Neurology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center
* These authors contributed equally

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Summary
Automatic Translation

The island assay is a relatively new, cost-effective assay that can be used to evaluate the basic locomotor behavior of Drosophila melanogaster. This manuscript describes algorithms for automatic data processing and objective quantification of island assay data, making this assay a sensitive, high-throughput readout for large genetic or pharmacological screens.

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Keywords: Drosophila Locomotor Behavior Island Assay High-throughput Analysis Automatic Data Processing Objective Quantification Movement Disorders Drug Testing Biological Replicates Image Data Analysis Experimental Setup Video Recording Image Processing
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