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3D Microtissues for injicerbare regenerativ terapi og høj overførselshastighed stof Screening
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Bioengineering
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JoVE Journal Bioengineering
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

3D Microtissues for injicerbare regenerativ terapi og høj overførselshastighed stof Screening

DOI:
10.3791/55982-v

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11:28 min

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October 04, 2017

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, , , , ,
1Department of Biomedical Engineering, School of Medicine,Tsinghua University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 3School of Life Sciences,Tsinghua University

Chapters

  • 00:05Title
  • 01:18Fabrication of 3D Microcryogels
  • 03:11Injectable 3D Microtissues for Treatment of Critical Limb Ischemia
  • 07:09Assembly of Microtissue Array Chip for High-throughput Drug Screening
  • 09:05Results: Microcryogels Used as a Therapeutic and for High-throughput Drug-screening
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver fabrikation af kombineret elastisk 3D makroporøs microcryogels ved at integrere microfabrication med cryogelation teknologi. Efter indlæsning med celler, er 3D microtissues genereret, som kan være let injiceres in vivo til at lette regenerativ terapi eller samlet i arrays for in vitro- høj overførselshastighed stof screening.

Tags

Regenerative TherapyDrug ScreeningCryogelationGelatinMicrocryogelsPMMA MicrostencilPDMS Ejector PinLyophilizationHigh-throughput

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