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3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
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Bioengineering
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JoVE Journal Bioengineering
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

DOI:
10.3791/55982-v

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11:28 min

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October 04, 2017

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, , , , ,
1Department of Biomedical Engineering, School of Medicine,Tsinghua University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 3School of Life Sciences,Tsinghua University

Chapters

  • 00:05Title
  • 01:18Fabrication of 3D Microcryogels
  • 03:11Injectable 3D Microtissues for Treatment of Critical Limb Ischemia
  • 07:09Assembly of Microtissue Array Chip for High-throughput Drug Screening
  • 09:05Results: Microcryogels Used as a Therapeutic and for High-throughput Drug-screening
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the fabrication of elastic 3D macroporous microcryogels by integrating microfabrication with cryogelation technology. Upon loading with cells, 3D microtissues are generated, which can be readily injected in vivo to facilitate regenerative therapy or assembled into arrays for in vitro high-throughput drug screening.

Tags

Regenerative TherapyDrug ScreeningCryogelationGelatinMicrocryogelsPMMA MicrostencilPDMS Ejector PinLyophilizationHigh-throughput

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