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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
 

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Article DOI: 10.3791/55996-v • 13:08 min • August 10th, 2017
August 10th, 2017 •
1, 1, 1, 2,3, 1
1School of Biological Sciences, Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing, National University of Singapore

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Summary
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The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.

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Keywords: Quantitative Localization Golgi Protein Fluorescence Mass HeLa Cells Transfection Nocodazole Fluorescent Beads Imaging Microscopy
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