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Journal / Genetics / High-resolution Melting PCR for Complement…
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Genetics

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JoVE Journal Genetics
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping
 

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Article DOI: 10.3791/56012-v • 07:26 min • July 18th, 2017
July 18th, 2017 •
1,2, 1,2, 1,2, 1,2, 3,4
1Department of Immunology, Reims University Hospitals, Robert Debré Hospital, 2Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, 3Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 4Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne

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Summary
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Here, we describe an innovative method to determine complement receptor 1 (CR1) length polymorphisms for use in several applications, particularly the assessment of susceptibility to diseases such as Alzheimer's disease (AD). This method could be useful to better understand the role of CR1 isoforms in the pathogenesis of AD.

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Keywords: Complement Receptor 1 Length Polymorphism High-resolution Melting PCR Alzheimer's Disease Genotyping Neuroscience DNA Extraction Proteinase K Lysis Buffer Ethanol Membrane Column
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