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Journal / Genetics / Amplification of Near Full-length HIV-1…
JoVE Journal
Genetics

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JoVE Journal Genetics
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
 

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Article DOI: 10.3791/58016-v • 10:18 min • October 16th, 2018
October 16th, 2018 •
1, 1, 1, 1
1Centre for Virus Research, The Westmead Institute for Medical Research, University of Sydney

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Summary
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Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

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Keywords: HIV-1 Proviruses Next-generation Sequencing Single-cell Amplification PCR Genetic Composition Replication Competent Cell Types Lysis Buffer Serial Dilution Positive Control Thermal Cycler
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