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用于异构蛋白复合物 - DNA相互作用研究的修饰的酵母一杂交系统
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
DOI:

10:47 min

July 24, 2017

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Chapters

  • 00:05Title
  • 00:40Preparation of Competent Cells
  • 02:40Co-transformation of Cells by Heat Shock
  • 04:39Transferring Cells onto New Plates
  • 05:25Preparing Culture for Measuring β-galactosidase Activity
  • 08:14Measuring β-galactosidase Activity
  • 09:07Results: Y1.5H Assay Detects Heteromeric Protein-DNA Interactions
  • 09:50Conclusion

Summary

Automatic Translation

这里描述的修饰的酵母单杂交测定法是经典酵母单杂交(Y1H)测定法的扩展,以研究和验证异源蛋白复合物 - DNA异构体系中任何功能基因组学研究的相互作用。

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