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用于异构蛋白复合物 - DNA相互作用研究的修饰的酵母一杂交系统
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Biochemistry
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Please note that all translations are automatically generated.
Click here for the English version.
用于异构蛋白复合物 - DNA相互作用研究的修饰的酵母一杂交系统
DOI:
10.3791/56080-v
•
10:47 min
•
July 24, 2017
•
Prateek Tripathi
,
José L. Pruneda-Paz
,
Steve A. Kay
1
Department of Neurology
,
University of Southern California
,
2
Section of Cell and Developmental Biology
,
University of California San Diego
Chapters
00:05
Title
00:40
Preparation of Competent Cells
02:40
Co-transformation of Cells by Heat Shock
04:39
Transferring Cells onto New Plates
05:25
Preparing Culture for Measuring β-galactosidase Activity
08:14
Measuring β-galactosidase Activity
09:07
Results: Y1.5H Assay Detects Heteromeric Protein-DNA Interactions
09:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里描述的修饰的酵母单杂交测定法是经典酵母单杂交(Y1H)测定法的扩展,以研究和验证异源蛋白复合物 - DNA异构体系中任何功能基因组学研究的相互作用。
Tags
Yeast-one Hybrid
Heteromeric Protein Complex
DNA Interaction
Functional Genomics
Protein-DNA Interactions
Co-transformation
TE Lithium Acetate
YPDA Medium
Competent Cells
Normalization Control
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