JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
从巨 Unilamellar 泡中提取膜纳米管
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
从巨 Unilamellar 泡中提取膜纳米管
DOI:
10.3791/56086-v
•
06:26 min
•
December 07, 2017
•
Coline Prévost*
1,2,3
,
Feng-Ching Tsai*
1,4
,
Patricia Bassereau
4
,
Mijo Simunovic
5
1
Laboratoire Physico Chimie Curie, Institut Curie
,
PSL Research University, CNRS UMR168
,
2
Department of Genetics and Complex Diseases, T. H. Chan School of Public Health
,
Harvard Medical School
,
3
Department of Cell Biology
,
Harvard Medical School
,
4
Sorbonne Universités
,
UPMC University Paris 06
,
5
Center for Studies in Physics and Biology
,
The Rockefeller University
Chapters
00:05
Title
00:50
Preparation of the Tube-pulling Experimental Chamber
02:32
Membrane Nanotube Pulling
05:12
Results: Representative Nanotube Formation Analyses
05:58
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
许多蛋白质在细胞感觉和导致膜曲度。我们描述了一种从脂泡中提取膜纳米管的方法, 以研究蛋白质或任何曲率-活性分子与弯曲膜的相互作用
体外
。
Tags
Membrane Nanotubes
Giant Unilamellar Vesicles
Protein-membrane Interactions
Endocytosis
Intracellular Trafficking
Lipid Membranes
Protein-shaping Membranes
Electroformation
Beta-casein
Aspiration Micropipette
Injection Micropipette
Streptavidin-coated Beads
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/56086?article_type=v
使用油脂介孔结构的测定结晶膜蛋白
check_url/56086?article_type=v
测量肽转运到大Unilamellar囊泡
check_url/56086?article_type=v
检查Bcl-2家族大单层脂质体的功能
check_url/56086?article_type=v
跨膜蛋白的重组,电压门控离子通道,KvAP,进入巨人单层囊的显微镜和膜片钳研究
check_url/56086?article_type=v
细胞调理介质细胞外囊泡的流式细胞学分析
check_url/56086?article_type=v
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
check_url/56086?article_type=v
Isolation of microRNAs from Tick
Ex Vivo
Salivary Gland Cultures and Extracellular Vesicles
check_url/56086?article_type=v
使用3D体积视图图像检测和定量隧道纳米管
check_url/56086?article_type=v
在研究细胞培养实验室中避免真菌和细菌污染的细胞培养技术和实践
check_url/56086?article_type=v
大
肠杆菌
重组蛋白填充囊泡的优化生产和分析
Read Article