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Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Cancer Research
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JoVE Journal Cancer Research
Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

DOI:
10.3791/56110-v

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12:25 min

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July 06, 2018

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, , ,
1Institute of Chemical Biology, Department of Chemistry,Imperial College London

Chapters

  • 00:05Title
  • 00:55Prepare Apparatus for Dispensing Addressable Droplets
  • 03:26Form Addressable Droplets and Load with Single Cells
  • 07:42Data Analysis
  • 09:39Results: Single Cell Data
  • 11:08Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.

Tags

Keywords: Single CellProtein Copy NumberAddressable Droplet MicroarrayHeterogeneityCentral DogmaGene ExpressionFused Silica TubingConcentric NozzlePTFE TubingFEP TubingHamilton SyringeCyanoacrylate GluePBSA Blocking Solution

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