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Anvendelse af kapsler til negativ farvning af virale prøver inden for biokonduktion
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Immunology and Infection
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JoVE Journal Immunology and Infection
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

Anvendelse af kapsler til negativ farvning af virale prøver inden for biokonduktion

DOI:
10.3791/56122-v

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08:22 min

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July 19, 2017

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, , , , , , , ,
1Pathology Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC

Chapters

  • 00:05Title
  • 01:08Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)
  • 01:45The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation
  • 04:24The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory
  • 05:50Results: Analyzing the Capsule Method of Negative Staining Viral Samples
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol giver vejledning til negative farvningsvirusprøver, der let kan bruges i BSL-2, -3 eller -4-laboratorier. Det omfatter brugen af ​​en innovativ behandlingskapsel, som beskytter transmissionselektronmikroskopi nettet og giver brugeren nemmere håndtering i de mere turbulente miljøer inden for biokonduktion.

Tags

VirusElectron MicroscopyNegative StainingBiocontainmentTEM GridsCapsulesGlutaraldehydeUranyl AcetatePotassium Phosphotungstic AcidOsmium TetroxideNanoparticles

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