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The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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Immunology and Infection
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JoVE Journal Immunology and Infection
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

DOI:
10.3791/53839-v

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07:27 min

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April 18, 2016

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,
1Viral and Rickettsial Diseases Department,Naval Medical Research Center

Chapters

  • 00:05Title
  • 00:40Prepare Plasmid DNA Dilutions as Standard for LAMP Reaction
  • 01:24Perform Standard LAMP Reaction
  • 02:54Perform LAMP Reaction with Reconstituted Reagents
  • 05:17Results: Detection of Coxiella burnetii with the LAMP Reaction
  • 06:37Conclusion

Summary

Automatic Translation

Automatic Translation

We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.

Tags

Lyophilized LAMP ReagentsCoxiella BurnetiiQ FeverDNA ExtractionLAMP ReactionPlasmid DNASerial Dilutions2x LAMP BufferBst DNA PolymeraseAgarose Gel ElectrophoresisReconstitution Buffer

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