Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Development of an in vitro model system for…
JoVE Journal
Immunology and Infection
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
 

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Article DOI: 10.3791/52222-v • 07:31 min • November 1st, 2014
November 1st, 2014 •
1, 2, 2
1Biological Department, King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield

Chapters

Summary
Automatic Translation

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Tags

Keywords: Equine IgE FcεRI In Vitro Model RBL-2H3.1 Cells Transfection Allergic Response β-hexosaminidase Release Diagnostic Tool Therapeutic Intervention
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter