Journal
/
/
개발<em> 체외</em의 상호 작용을 연구> 모델 시스템<em> 에쿠스</em><em> caballus</em높은 친 화성 수용체 FcεRI에>의 IgE
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
DOI:

07:31 min

November 01, 2014

, ,

Chapters

  • 00:00Title
  • 00:10Introduction
  • 01:14Animation
  • 02:37Washing
  • 03:22Antigen Challenge
  • 03:48Substrate
  • 04:23Stopping the Reaction
  • 04:47Reading
  • 04:54Calculations
  • 06:22Results
  • 07:02Conclusion

Summary

Automatic Translation

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Related Videos

Read Article