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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
JoVE Journal
Biology
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JoVE Journal Biology
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
DOI:

10:58 min

December 23, 2017

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Chapters

  • 00:00Title
  • 01:03Identification, Isolation, and Cloning of Phaseolus vulgaris NIN Promoter
  • 05:11Agrobacterium rhizogenes Transformed Hairy Roots of Phaseolus vulgaris
  • 09:44Results: NIN Promoter Expression in Root Nodules of Phaseolus vulgaris
  • 10:21Conclusion

Summary

Automatic Translation

Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.

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