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Journal
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Biology
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植物启动子分析: 菜豆根瘤特异启动子的鉴定与表征
JoVE Journal
Biology
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JoVE Journal
Biology
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Please note that all translations are automatically generated.
Click here for the English version.
植物启动子分析: 菜豆根瘤特异启动子的鉴定与表征
DOI:
10.3791/56140-v
•
10:58 min
•
December 23, 2017
•
Kalpana Nanjareddy*
1
,
Manoj-Kumar Arthikala*
1
,
Alma-Leticia Aguirre
,
Brenda-Mariana Gómez
,
Miguel Lara
1
Ciencias Agrogenómicas
,
Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM)
,
2
Instituto de Biología, Universidad Nacional Autónoma de México (UNAM)
,
Ciudad Universitaria, Coyoacan
Chapters
00:00
Title
01:03
Identification, Isolation, and Cloning of
Phaseolus vulgaris
NIN Promoter
05:11
Agrobacterium rhizogenes
Transformed Hairy Roots of
Phaseolus vulgaris
09:44
Results: NIN Promoter Expression in Root Nodules of
Phaseolus vulgaris
10:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
启动子表达分析对于提高对基因调控的理解和靶基因的时空表达至关重要。在这里, 我们提出了一个协议, 以确定, 隔离和克隆植物启动子。此外, 我们还描述了在菜豆多毛根中的根瘤特异启动子的特性。
Tags
Plant Promoter
Root Nodule
Hairy Root
Phaseolus
Reporter Gene
Agrobacterium Rhizogenes
NIN Gene
PCR
Gateway Cloning
Binary Vector
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