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植物启动子分析: 菜豆根瘤特异启动子的鉴定与表征
JoVE Journal
Biology
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JoVE Journal Biology
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
DOI:

10:58 min

December 23, 2017

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Chapters

  • 00:00Title
  • 01:03Identification, Isolation, and Cloning of Phaseolus vulgaris NIN Promoter
  • 05:11Agrobacterium rhizogenes Transformed Hairy Roots of Phaseolus vulgaris
  • 09:44Results: NIN Promoter Expression in Root Nodules of Phaseolus vulgaris
  • 10:21Conclusion

Summary

Automatic Translation

启动子表达分析对于提高对基因调控的理解和靶基因的时空表达至关重要。在这里, 我们提出了一个协议, 以确定, 隔离和克隆植物启动子。此外, 我们还描述了在菜豆多毛根中的根瘤特异启动子的特性。

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