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Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
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Cancer Research
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JoVE Journal Cancer Research
Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

DOI:
10.3791/56248-v

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08:13 min

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December 07, 2017

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, , ,
1Cancer Science Institute of Singapore,National University of Singapore, 2Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore

Chapters

  • 00:05Title
  • 00:38siRNA Transient HIV-Tat-interactive Protein 60 kDa (TIP60) Depletion
  • 02:08Wound-healing Assay Cell Seeding and TIP60 Knockdown Efficiency Evaluation
  • 04:10Wound Creation, Imaging, and Single Cell Movement Tracking
  • 06:32Results: Representative Videos and Quantifications of siTIP60 and siControl Cell Migration
  • 07:49Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.

Tags

Live-imagingBreast Epithelial CellCell MigrationTIP60 DepletionCancer MetastasisCell MorphologySIRNA TransfectionCell CultureCell CountingCell SeedingGene Knockdown Efficiency

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