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En metode til at vurdere Fc-medieret effektor funktion induceret af Influenza Hemagglutinin specifikke antistoffer
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

En metode til at vurdere Fc-medieret effektor funktion induceret af Influenza Hemagglutinin specifikke antistoffer

DOI:
10.3791/56256-v

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04:47 min

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February 23, 2018

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, , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease,J. Craig Venter Institute

Chapters

  • 00:05Title
  • 00:42Influenza Virus Hemagglutinin Expression
  • 02:18Antibody-dependent Cell-mediated Cytotoxicity (ADCC) Analysis
  • 04:01Results: Representative ADCC Analysis
  • 04:24Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en metode til at måle aktivering af Fc-medieret effektor funktion af antistoffer der er rettet mod influenza virus hemagglutinin. Denne analyse kan også tilpasses for at vurdere monoklonale antistoffer eller polyklonale sera rettet mod andre virale overflade glykoproteiner evne til at fremkalde Fc-medieret immunitet.

Tags

Keywords: Fc-mediated Effector FunctionsInfluenza HemagglutininAntibodyADCCCell-mediated CytotoxicityTransfectionInfectionA549 Cells293 CellsVirusImmunologyVaccinology

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