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Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
 

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Article DOI: 10.3791/56277-v • 09:23 min • September 24th, 2017
September 24th, 2017 •
1, 1, 2, 2, 1, 2,3, 2, 1
1Lundbeck Foundation Research Initiative on Brain Barriers and Drug Delivery, Department of Biomedicine, Aarhus University, 2Institute of Pharmaceutical Science, King's College London, 3HICoE Centre for Drug Research, Universiti Sains Malaysia

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Summary
Automatic Translation

The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.

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Keywords: Blood-brain Barrier Porcine Brain Endothelial Cells In Vitro Model Primary Cell Isolation Tissue Homogenization Capillary Isolation Cell Digestion Cell Culture TEER Permeability
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