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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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Genetics
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JoVE Journal Genetics
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

DOI:
10.3791/56286-v

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06:40 min

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March 22, 2018

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, ,
1Division of Clinical Research,Fred Hutchinson Cancer Research Center, 2Departments of Medicine and Biochemistry,University of Washington

Chapters

  • 00:04Title
  • 00:44Preparing Cells and Sorting Using Flow Cytometry
  • 03:13DNA Extraction and Sequencing Library Preparation
  • 05:20Results: Late Replicating Sites in Budding Yeast
  • 06:14Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a technique for combining flow cytometry and high throughput sequencing to identify late replicating regions of the genome.

Tags

Keywords: G2-seqHigh-throughput SequencingDNA ReplicationCell CycleLate Replicating RegionsGenomeYeastFlow CytometryCell SortingDNA ContentG1SG2 Phases

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