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Journal / Genetics / Promoter Capture Hi-C: High-resolution,…
JoVE Journal
Genetics

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JoVE Journal Genetics
Promoter Capture Hi-C
 

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Article DOI: 10.3791/57320-v • 10:16 min • June 28th, 2018
June 28th, 2018 •
*1, *1,2, 1,3, 1,4, 1,5
1Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute, Babraham Research Campus, 5Department of Biological Science, Florida State University
* These authors contributed equally

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Summary
Automatic Translation

DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.

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Keywords: Promoter Capture Hi-C Genome-wide Profiling Promoter Interactions Gene Regulatory Elements Target Genes Genome Structure Gene Expression Control Noncoding Disease Variants Regulatory Control Sequences Cell Lysis Restriction Buffer Cell Nuclei
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