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Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
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Chapters
- 00:04Title
- 00:33Cell Transfection
- 02:08Rh123 Labeling
- 04:21Detection of the Mitochondrial Membrane Potential (MMP)
- 06:29Confocal Laser Scanning Fluorescence Microscope
- 08:34Statistical Analysis
- 09:48Results: Detection of Mitochondrial Membrane Potential via Normal Fluorescent Microscopy and Confocal Laser Scanning Microscopy
- 11:53Conclusion
Here we present a detailed protocol for the application of rhodamine 123 to identify the mitochondrial membrane potential (MMP) and study CLIC4 knockdown-induced HN4 cell apoptosis in vitro. Under common fluorescence microscope and confocal laser scanning fluorescence microscope, the real-time change of the MMP was recorded.
