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Cancer Research
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应用肺转移法研究骨肉瘤转移性肺定植的实用思考
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Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
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应用肺转移法研究骨肉瘤转移性肺定植的实用思考
DOI:
10.3791/56332-v
•
07:44 min
•
March 12, 2018
•
Michael M. Lizardo
2
,
Poul H. Sorensen
3
1
Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute
,
National Institutes of Health
,
2
BC Cancer Agency
,
Provincial Health Services Authority
,
3
Department of Pathology and Laboratory Medicine
,
University of British Columbia
Chapters
00:04
Title
00:52
Tail Vein Injection and Lung Insufflation
02:48
Widefield Fluorescence Lung Slice Imaging and Analysis
05:49
Results: Representative Lung Slice Imaging Analysis
06:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文的目的是为肺转移试验 (美洲狮) 的协议提供详细的描述。该模型允许研究人员使用 widefield 荧光或共聚焦激光扫描显微镜研究肺组织中的转移性骨肉瘤 (OS) 细胞生长。
Tags
Metastatic Lung Colonization
Osteosarcoma
Pulmonary Metastasis Assay
Trachea Cannulation
Agarose Insufflation
Three-dimensional Microenvironment
Tumor Cell Visualization
Lung Slices
Ex Vivo Culture
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