/

Cancer Research

/

应用肺转移法研究骨肉瘤转移性肺定植的实用思考

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Please note that all translations are automatically generated. Click here for the English version.

应用肺转移法研究骨肉瘤转移性肺定植的实用思考

DOI:

10.3791/56332-v

•

07:44 min

•

March 12, 2018

•

Michael M. Lizardo², Poul H. Sorensen³

¹Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health, ²BC Cancer Agency,Provincial Health Services Authority, ³Department of Pathology and Laboratory Medicine,University of British Columbia

Chapters

00:04Title
00:52Tail Vein Injection and Lung Insufflation
02:48Widefield Fluorescence Lung Slice Imaging and Analysis
05:49Results: Representative Lung Slice Imaging Analysis
06:44Conclusion

Summary

Automatic Translation

本文的目的是为肺转移试验 (美洲狮) 的协议提供详细的描述。该模型允许研究人员使用 widefield 荧光或共聚焦激光扫描显微镜研究肺组织中的转移性骨肉瘤 (OS) 细胞生长。

Tags

Metastatic Lung Colonization Osteosarcoma Pulmonary Metastasis Assay Trachea Cannulation Agarose Insufflation Three-dimensional Microenvironment Tumor Cell Visualization Lung Slices Ex Vivo Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

肝大肠癌转移的高级动物模型：成像技术和转移性克隆性质

肺定植法在肺部组织循环肿瘤细胞可视化中的建立

应用 Fraumeni 综合征诱导多潜能干细胞建模骨肉瘤

脱细胞和细胞肺模型研究肿瘤转移

循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

结合使用尾静脉转移分析与实时 Vivo 成像，以量化乳腺癌转移殖民化和肺负担

肿瘤细胞外侧尾静脉注射后肺转移的病理分析

利用计算机图像分析提高4T1乳腺癌模型中肺转移的定量

同源原位骨肉瘤Sprague Dawley大鼠模型，截肢控制转移率

研究癌症转移至肺部的永久窗口

Read Article