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肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募
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Cancer Research
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JoVE Journal Cancer Research
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
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肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募

DOI:
10.3791/56333-v

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10:59 min

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November 23, 2017

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1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics,University of Southern California, 2The Saban Research Institute of Children’s Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine,University of Southern California

Chapters

  • 00:05Title
  • 01:09Generation of Lentivirus Particles
  • 02:34Generation of Stably Transfected Lines
  • 04:55Isolation of Monocytes from Human Blood
  • 08:00Boyden Chamber Migration Assay
  • 09:25Results: PAI-1 Promotes Macrophage Migration
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

本议定书作为一个计划, 建立一个功能性的癌症细胞系的系统, 并随后使用, 特别是研究肿瘤细胞源性蛋白质在招募单核/巨噬细胞到肿瘤微环境的作用。

Tags

Conditional Gene KnockdownCancer Cell LinesMonocyte RecruitmentMacrophage RecruitmentTumor MicroenvironmentLentiviral TransductionHEK-293 CellsHCT-116 CellsMDA-MB-231 CellsMonocyte PurificationPuromycin Selection

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