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Neuroscience

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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
 

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Article DOI: 10.3791/56358-v 10:24 min December 3rd, 2017
December 3rd, 2017

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Summary

This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

Tags

Keywords: Mouse Embryonic Stem Cells Cortical Interneuron Progenitors Nkx2.1 Parvalbumin Somatostatin MEF Feeder Cells Trypsin-EDTA Gelatin-coated Plates KSR-N2 Media Embryoid Bodies
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