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Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

DOI:
10.3791/2373-v

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15:40 min

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January 24, 2011

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, , ,
1Department of Anatomy,University of Wisconsin-Madison

Chapters

  • 00:00Title
  • 02:23Construction of Imaging Chambers
  • 03:45Cortical Glial Feeder Layer Preparation for Long-term Cultures
  • 07:39Cortical and/or Hippocampal Dissection and Electroporation
  • 12:10Representative Results
  • 14:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

Tags

NucleofectionPrimary CultureEmbryonic MouseHippocampal NeuronsCortical NeuronsCNS Neuronal PolarizationAxon/dendrite OutgrowthSynapse FormationGlial ConditioningTransfectionElectroporationDNAFluorescently-tagged Fusion ProteinsProtein DynamicsAxon OutgrowthBranchingProtein LocalizationCNS Development

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