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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
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Neuroscience
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JoVE Journal Neuroscience
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

DOI:
10.3791/52475-v

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08:45 min

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February 19, 2015

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, , ,
1Division of Brain Sciences, Department of Medicine,Imperial College London, 2Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Chapters

  • 00:05Title
  • 01:29Making Preparations
  • 02:45Dissecting the Embryonic Ventral Midbrain
  • 04:44Dissociating the Ventral Midbrain Cells
  • 06:09Plating and Culturing the Ventral Midbrain Cells
  • 07:49Results: Characterizing the Dopaminergic Neuron Cultures
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

Tags

Primary Mesencephalic Dopaminergic NeuronsEmbryonic Rodent BrainsParkinson’s DiseaseNeurodegenerative DisorderCell CultureMidbrain Dopaminergic NeuronsE12.5 MouseE14.5 RatNeuronal MorphologyPhysiological Characteristics

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