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Vurdering af blod - hjerne barrieren permeabilitet af intravenøs Infusion af FITC-mærket Albumin i en musemodel af neurodegenerativ sygdom
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
DOI:

07:22 min

November 08, 2017

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Chapters

  • 00:05Title
  • 01:10Surgical Procedure for FITC-albumin Infusion into the Jugular Vein
  • 03:57Histological Sectioning of Brain Infusion with FITC-albumin
  • 04:47Fluorescence Microscopy and Acquisition of Color Images
  • 05:44Results: FITC-albumin Extravasation as a Marker of Blood-brain Barrier Damage
  • 06:44Conclusion

Summary

Automatic Translation

I denne undersøgelse præsenterer vi en let og effektiv procedure til evaluering af forstyrrelser af blod - hjerne barrieren neurodegenerative betingelser. For at nå vores mål, vi infunderes høj molekylvægt fluorescein isothiocyanat mærket (FITC)-albumin i mus jugularis vene og vurderet dets lækage i hjernen parenkym af Fluorescens mikroskopi.

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